HPLC Potency Testing

HPLC Machine

General Information

We use high-performance liquid chromatography (HPLC) to determine the relative quantities of molecules in your sample(s). HPLC instruments are used to quantify a large assortment of different compounds, however, our system is specifically designed to detect cannabinoids. HPLC works by measuring how quickly the components in a sample (THC, CBD, etc.) move through a special column. The time it takes for each component to move through the column is called its residence time. We are able to identify and quantify components in a sample by comparing residence times of a sample with residence times of a ‘standard sample’ containing known amounts of the various cannabinoids.

THC and CBD are the most important cannabinoids for most growers: THC levels must be kept below 0.3% to comply with state and federal laws and CBD levels should be maximized to ensure maximum profits for the grower. These are, however, in competition. Harvesting early means low THC levels but also minimal CBD levels. Harvesting late will mean higher THC levels (and risking a non-compliant crop). The only way to know the best time to harvest is by using potency testing. We recommend getting multiple tests of your plot over the course of five weeks leading up to harvest. This allows us to develop a THC/CBD profile and gives the grower an idea of where THC/CBD levels are headed with respect to time.

Pricing

 Number of Samples Price per Sample
1-4 $75

5-15

$60

16-30

$50

> 30

Call/Email to Inquire

When purchasing a 5-sample package, 5 samples from different plants can be sent in at the same time, or 5 samples from the same plant can be sent in at 5 different times. You get 5 total tests, and it is your decision when you want to send in the samples. Tests do carry over into the next year if they are unused. Please contact us if you have a large number of samples that you would like tested. We can work together on a special deal for bulk sampling.

Molecules Tested For Potency

Our standard testing services will test for CBN, CBD, THC, THCa, and CBDa. If you are interested in potency testing for additional cannabinoids, please inquire for pricing.

Preparing a sample

 **Please include your DATCP Hemp Growing License or Fit For Commerce Certificate with all samples**

We handle most of the work required to prepare a sample for analysis. Whether it be plant material, oil extract, or isolate, there are only a few simple steps you have to take before sending us your sample(s):

Sample Type: Plant Material

  1. Collect ~2 grams of plant material from the part of the plant you’d like tested. For most, this will be the flower portion of the plant. Please remove all non-flower aspects of plant or all aspects you do not wish to have tested (e.g. stems, leaves, etc.).
  2. Place sample in a ziplock bag. One zip-locked bag must be used for each different sample. In other words, 5 different zip-locked bags must be used if you are sending in 5 samples at a time.
  3. Label each bag using whatever labeling scheme you desire, and results will be returned corresponding to your chosen labeling scheme.
  4. Mail to our address, or drop your samples off at our office during normal business hours.

Notes: Fresh samples should not be dried. We will handle this aspect of sample preparation to ensure quality results. However, flower harvested and properly dried is satisfactory for submission. Also, if you’d like to collect samples over the course of a few days and send them in all at once, we recommend keeping samples in the fridge until shipment.

Sample Type: Hemp/CBD Oil or CBD isolate

  1. Obtain glass vials or test tubes to send your samples in.
  2. Place ~1 gram of oil/isolate in a glass vial or test tube. One glass vial/test tube must be used for each different sample. In other words, 5 different vials/tubes must be used if you are sending in 5 samples at a time.
  3. Label each vial/tube using whatever labeling scheme you desire, and results will be returned corresponding to your chosen labeling scheme.
  4. Ensure that the glass vials are secured in such a way that they will not break in the mail. Gently wrapping a paper towel around each vial is a good way to prevent any of them from breaking.
  5. Mail to our address, or drop your samples off at our office during normal business hours.

Certificate of Analysis

Upon completion of the testing, we provide the customer with a Certificate of Analysis (COA) indicating the cannabinoid levels of their sample. Our typical turnaround time is two business days from receiving the sample. Below is an example of one of our COAs. Most buyers will require a COA before purchasing material, however, these cannot be used to pass the DATCP end of season test. 

 

Mailing Address

WI Hemp Scientific

N63W22595 Main St.

Sussex, WI 53089